Inama yoroshye yukuntu wakwirinda kwanduza mugihe cya PCR

Tekinoroji ya PCR nubuhanga bwo kongera aside nucleique yigana inzira yo kwigana ADN muri vitro.Irasubiramo cyane cyane inzinguzingo eshatu: gutandukanya ubushyuhe bwo hejuru, ubushyuhe buke hamwe no kwaguka, hanyuma ikongerera ADN intego inshuro miriyoni.Rero, tekinoroji ya PCR ifite ibyiza byo kumva cyane.Nyamara, hari impande ebyiri kuri buri giceri, kandi imbogamizi nini yikoranabuhanga rya PCR ni uko ishobora kwanduzwa cyane, kandi umubare muto cyane wanduye ushobora gukurura ibyiza.

Laboratoire nucleic imaze kwandura, ubushakashatsi busanzwe bugomba guhagarara.Ibikoresho byingenzi hamwe nabakozi birasabwa kugeza igihe habonetse isoko yanduye, cyangwa isuku ya laboratoire ikaba isanzwe.Byongeye, raporo ya laboratoire igomba guteshwa agaciro.Ibisubizo byizewe nyuma yo kongera kugerageza.

Kugirango twirinde neza kwanduza no kubona ibisubizo byizewe kandi byizewe, dukeneye gufata ingamba zimwe na zimwe zo gukumira cyangwa gukuraho umwanda mubikorwa.

icyapa

Inkomoko yo kwanduza.
Kwanduza
Nka kontineri ya reagent, imiyoboro, amazi nibindi bisubizo birashobora kwanduzwa na acide nucleic mugihe cyo gutegura reagent ya PCR.

Kwanduza ibikoresho
Semi-automatic nucleic aside ikuramo hamwe na acide nucleic acide yuzuye irashobora gutera ingero cyangwa inyandikorugero ya acide nucleic isuka mugihe cyo kuyikuramo no kwanduza imashini.Cyangwa ikinini kiri mubikoresho ntigisukurwa neza kandi amazi asigara cyangwa kristu isigara ihumeka kugirango ikore aerosole, nayo ishobora gutera umwanda.

Kugereranya kwanduzanya
Kugereranya kwanduzanya bishobora guterwa nimpamvu zitandukanye: kwanduza ibikoresho byakusanyirijwe hamwe;isuka rya kontineri kubera gufunga nabi mugihe icyitegererezo gishyizwe;ingero zifatiye hanze yikintu;kwanduza impemu na aerosole mu kirere.By'umwihariko, virusi zirashobora gukwirakwira mu kirere cyangwa mu gukora aerosole, bikaviramo kwanduzanya.

PCR yanduye
Iki nikibazo gikunze kwanduzwa muri PCR.Bikunze kuboneka mubisubizo bisaba gufungura nyuma yo kwongerwaho cyangwa gufata nabi ibicuruzwa nyuma yo kwongera.Ni ukubera ko ingano ya kopi yibicuruzwa bya PCR ari nini cyane kuruta ntarengwa ya kopi nkeya zagaragajwe na PCR.Kubwibyo, umubare muto cyane wibicuruzwa bya PCR bishobora kwanduza ibyiza.

Cloni Yanduye
Ibyiza bifatika bikoreshwa mubikorwa bya laboratoire, kandi ibyinshi muribi bituruka kuri plasima zimwe na zimwe.Ubwinshi bwa plasima ya clonide kuri buri gice ni kinini cyane kandi birashobora kwanduzwa byoroshye iyo bidakoreshejwe neza.

Laboratoire zimwe zikoresha plasmide yazo nkibicuruzwa bigenzura ubuziranenge.Ariko, nyuma yo kwitegura, imyanda ntabwo ikorwa muburyo bukomeye bihagije, biganisha ku kwanduza laboratoire na plasmide bityo ingero.

Ingamba zo kurwanya umwanda
Kugabana ibice
Gutandukanya gushyira mu gaciro kwa laboratoire.Kugabanya cyangwa kugabana imikorere yintambwe zikurikira: gutunganya ingero, gutegura igisubizo cya PCR, kongera imbaraga za PCR, kumenyekanisha ibicuruzwa bya PCR, nibindi. Byongeye kandi, hakwiye kwitabwaho cyane cyane ko gutunganya ibicuruzwa no kumenyekanisha ibicuruzwa bya PCR bigomba gutandukana na izindi ntambwe.

Nibyiza kubigabanyamo: ahantu ho gutunganyirizwa, agace ka PCR gategura igisubizo, agace ka PCR cyongerewe imbaraga, hamwe na PCR imenyekanisha ibicuruzwa.Kandi ibikoresho byabo bya laboratoire hamwe nabifuza bagomba gukoreshwa wenyine.

UV ikoreshwa muri laboratoire igomba guhagarikwa mbere yubushakashatsi bwo gusenya ADN isigaye cyangwa RNA.

Ingingo kuri buri karere ntigomba kuvangwa
Buri gace ka laboratoire kagomba gushyirwaho neza nibikoresho bitandukanye nibintu bitandukanye mumabara agaragara kugirango birinde kuvanga ibikoresho nibintu biva mubikorwa bitandukanye.Kurugero, imyenda y'akazi, inkweto z'akazi, amabati, imyanda, imyenda, amakaramu, n'ibindi bikoreshwa muri buri gace.

Koresha imiyoboro ya pipette ikoreshwa, tebes ya centrifuge, gants zitagira umukungugu, masike, nibindi.

Gushiraho uburyo busanzwe bwo guhumeka no guhumeka
Koresha uburyo bwuzuye bwo gutanga no guhumeka sisitemu yo guhumeka igihe cyose bishoboka.Icyerekezo gikomeye cyo gutemba kirasabwa mugihe cyo kugera kuri buri gice cyakorerwagamo, ni ukuvuga gutegura reagent hamwe nububiko → ahantu ho gutegurira icyitegererezo area Agace kongererwa PCR area agace kasesenguye ibicuruzwa, nta retrograde itemba.

Gukurikirana umwanda
Kugenzura umwanda bikorwa hifashishijwe ingamba zikurikira: gushyiraho igenzura ryiza kandi ribi, gusubiramo ibizamini, guhitamo primers mu turere dutandukanye kugirango PCR yongere, nibindi, bityo ifate ingamba zo gukumira no gukuraho umwanda.

Isuku ya laboratoire isanzwe
Tegura igisubizo cyangiza kirimo 0.5% ya chlorine ikora kugirango uhanagure hejuru yibikoresho, ahabigenewe, hasi, hejuru yinyandiko, agasanduku kanyuze, inzugi nidirishya, hanyuma ushiremo imyanda imbere mubikoresho.

Inzoga ziterwa no guhumeka ikirere zikorwa ukurikije hejuru-hasi, imbere-hanze.

Ihanagura imiyoboro, umunani-centrifuges, imashini ivanga nibindi bikoresho bito hamwe n'inzoga (75%).

Ihanagura ibikoresho hejuru yububiko, hasi na etage hamwe namazi meza kandi koza ibikoresho byamazi hamwe namazi meza.

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